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The ikra pipeline automated the RNA-seq data analysis process, including read quality control (Trim Galore version 0.6.6 (Babraham Bioinformatics, UK 34 with Cutadapt 35 and transcript quantification (Salmon version 1.4.1 using reference transcript sets in GENCODE release 31 for humans)
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Parameters of spermatozoa and serum carnitine levels in this study
These metabolites intervene in retinal cell apoptosis from aspects such as immune regulation, antioxidant activity, anti-inflammation, autophagy regulation, and mitochondrial function protection and can deal with the complex disease mechanisms more comprehensively
The group of wild-type mice treated with hepsin and a reduced dose of 200 mg/kg NAC exhibited significantly lower levels of both enzymes, indicating reduced liver damage compared to the other groups (Fig